Brock Biology Of Microorganisms 14th Edition By Madigan – Test Bank

Chapter 11 Genetic Engineering and Biotechnology

Multiple Choice Questions

1) If a foreign gene is cloned into an expression host, it is important that the host itself
A) not produce the protein being studied.
B) produce the protein in larger amounts than the vector.
C) repress the genetic expression being studied.
D) produce signal proteins to tag the host protein.
Answer: A
Bloom’s Taxonomy: Comprehension
Chapter Section: 11.9

2) Detecting a specific protein with an antibody is considered a(n) ________ method.
A) selection
B) screening
C) isolation
D) duplication
Answer: B
Bloom’s Taxonomy: Knowledge
Chapter Section: 11.4

3) To discover a catabolic gene cluster, cloning large sequences of approximately 40 kbp requires the utility of
A) bacterial artificial chromosomes (BACs).
B) cosmids or fosmids.
C) a eukaryotic host to house the large foreign DNA.
D) multiple gene fusions.
Answer: B
Bloom’s Taxonomy: Application
Chapter Section: 11.10

4) Expression vectors are designed to ensure that ________ can be efficiently ________.
A) mRNA / transcribed
B) DNA / transcribed
C) mRNA / translated
D) DNA / translated
Answer: A
Bloom’s Taxonomy: Comprehension
Chapter Section: 11.9

5) A(n) ________ gene is a gene that encodes a protein that is easy to detect and assay.
A) encoder
B) translational
C) reporter
D) recorder
Answer: C
Bloom’s Taxonomy: Knowledge
Chapter Section: 11.6
6) One of the more formidable obstacles to mammalian gene cloning is the presence of
A) introns.
B) exons.
C) repressors.
D) integrators.
Answer: A
Bloom’s Taxonomy: Knowledge
Chapter Section: 11.11

7) Which of the following is NOT a characteristic of a type II restriction endonuclease?
A) cleavage product can be either blunt or sticky ended but is always the same for an individual enzyme
B) recognizes a specific palindromic site for cleavage
C) recognition site length varies among enzymes but is always the same for an individual enzyme
D) unable to cleave methylated DNA
Answer: D
Bloom’s Taxonomy: Comprehension
Chapter Section: 11.1

8) What type of vector can replicate and be maintained stably in two (or more) unrelated host organisms?
A) virus
B) expression
C) shuttle
D) integrating
Answer: C
Bloom’s Taxonomy: Knowledge
Chapter Section: 11.9

9) The genes encoding luciferase, green fluorescent protein (GFP), and β-galactosidase are typically used in cloning as
A) transcription regulators.
B) global control genes.
C) promoter sequences.
D) reporter genes.
Answer: D
Bloom’s Taxonomy: Application
Chapter Section: 11.6

10) To verify a gene was cloned into a vector successfully, sequencing the vector as well as ________ are commonly performed.
A) agarose gel electrophoresis
B) fluorescence in situ hybridization
C) protein purification
D) northern blots
Answer: A
Bloom’s Taxonomy: Application
Chapter Section: 11.1